Bd accuri c6 flow cytometry system available for sale. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Bd csampler software user guide iv 782009901 rev2 table of figures figure 11. Start the c6 flow cytometer, open bd accuri c6 software and wait for the green traffic light message. The bd accuri c6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. When setting up the cytometer, note the details in the following sections to maximize the instruments performance.
By default, bd accuri c6 software is set to a primary threshold of channel 80,000 on fsch. Buy from accuri, or make your own citric acid solution. Accuri c6 maintenance cleaning and decontamination alternatives. Warning failure to keep the mat clear of plates, tubes or any other materials may result in damage to the bd csampler.
The facsverse is a far superior instrument to the c6. Bd accuri c6 flow cytometer instrument manual 7820018 rev2 3 refer to the bd accuri c6 safety and limitations manual pn 7820091 for complete safety information. Many bd accuri c6 customers can begin collecting and analyzing data with the help of a quick start guide. The bd csampler will auto matically align itself and then remain in the home position figure 8 9.
If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. Accuri cytometers cflow user guide usf biological sciences. For information on using bd accuri c6 software, see the bd accuri c6 software. Our neuroimmunology lab currently has a bd accuri c6 that has broken about every year and a half. The flow rate, relative to sample concentration, should minimize doublets and larger. Fcs express will import all of the experiment information from the bd accuri c6 software files including all plots on the collect and analyze tab, the native high resolution 24 bit fcs data, all gates, gating hierarchies, markers, quadrants, plot formatting and scaling settings. Take a leap in flow cytometry with the bd accuri c6 plus. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results. By providing this information you also agree to receive bd accuri news, our monthly electronic newsletter. Platforms referred to as bd accuri represent both the bd accuri c6 plus and bd accuri c6. Accuri c6 maintenance cleaning and decontamination.
It uses a bunch of new technology that bd has built to address usability software and. Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3. The bd accuri c6 software importer allows you to import your entire experiment from accuri c6 and c6 plus systems. Easytouse software with automated qc and application templates bd accuri c6 plus software features an intuitive user interface. Default cytometer configuration for the accuri c6 analyzer. To fill the cleaning solution bottle green line, add 3 ml of 1030% citric acid and fill with di. Cflow software refers generically to either cflow or cflow plus software. A new workspace can be used to create an analysis template and to collect a new dataset. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Bd accuri c6 flow cytometer quick set up guide umces. The intuitive interface of the software guides the user through workflows. Facsdiva v6 software overview course bd biosciences accuri c6 software user guide.
Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient to cover the inlet tubing and that there are no kinked fluidic lines. The bd accuri c6 software installer is on the cd that came with the c6. Optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order. Threshold settings can be changed before, during, or after data acquisition. Feb 20, 2017 bd accuri c6 flow cytometry system available for sale.
Technical bulletin a guide to absolute counting using the bd accuri c6 flow cytometer bd biosciences august 2011 the ideal cell concentration range for accurate counting varies among cell types, based on considerations such as size, shape, and tendency to clump. Even new flow cytometry users find it so easy to learn and use that. The templates have been designed using the current bd accuri c6 software, version 264. Learn about bd life sciences diagnostic systems bd bbl crystal mind software. Bd life sciences diagnostic systems bd bbl crystal mind.
Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. After contracts terminate, bd will support the bd accuri c6 system for as long as reasonably possible, depending on parts availability and other matters. It also includes common troubleshooting help and service information. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. Bd biosciences bd accuri c6 plus community, manuals and. Instead, prompt windows to look for the missing device driver and point it to the newly installed bd accuri software folder. If you have any questions regarding this matter, please see the following bd accuri frequently asked questions or contact bd service contracts administration 1. Bdaccuri c6 flow cytometer system bdaccuri c6 flow cytometer system includes bd accuri c6 software instrument,generating statistics, analyzingresults. Tabbed views for collection, analysis, and statistics. Press the power button on the bd accuri c6 instrument face panel. Bd accuri c6 plus software features an intuitive user interface. Performance validation of the bd accuri c6 was then done.
The accuri cflow or cflow plus software cflow allows you to control the c6 flow cytometer system to acquire data, generate statistics, and analyze results. Bd csampler software user guide iv 782009901 rev0 table of figures figure 11. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. Breakthrough with the bd accuri c6 plus cell analyzer. Click here to view the bd fluorescence spectraviewer. Operate the instrument as described in this instruction manual. In addition, c6 has a flow metering system for automatic calculation of absolute cell counts. Install, update, and run antivirus protection software on a regular basis. If you are interested in using a fluorochrome that is not listed here with the accuri c6, please contact the operator. Only operate the accuri c6 flow cytometer with an original ed version of software authorized by accuri. Bd accuri c6 flow cytometer instrument manual bd biosciences. Collect the collect tab is used to collect or import a sample, configure the run settings and set up plots and gates. Please complete the following information to request a demonstration of the bd accuri c6 plus flow cytometer.
Bd accuri cytometers use the cflow software, which produces fcs 3. Turn on the computer and log in with your ucf info. The software for accuri c6 cflow plus is very userfriendly and efficient. Using the bd accuri c6 cytometer for rapid and accurate. Nov 09, 2017 optimize your flow cytometry best practices for sample preparation, staining and analysis duration. Bd accuri c6 with plate handler april 2016 this protocol contains instructions for setting up an accuri c6 with plate handler. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Bd accuri c6 plus software files can be exported in fcs 3. All reagents and kits are compatible with both the bd accuri c6 plus and bd accuri c6 flow cytometer systems. Even new flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. Msds linked at the right cleaning solution, kr225 citric acid. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. Daniels instructions below on how to go about this were invaluable. This portable and compact system holds the analytical power of a cytometer twice its size.
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